It is an aminoglycoside antibiotic and a narrow-spectrum antibiotic. It exerts potent antibacterial activity against most Gram-negative bacilli such as Escherichia coli, Proteus, Salmonella and Pasteurella multocida. Staphylococcus aureus and mycobacteria are also relatively sensitive to it. Pseudomonas aeruginosa, Gram-positive bacteria (except Staphylococcus aureus), rickettsiae, anaerobic bacteria, fungi and other microorganisms are usually not sensitive to kanamycin.